Test card

Y-Malachite green speed test reagent

Application

A. It is widely used to detect various types of aquarium, ponds and other water, whether it contains malachite green ingredients. B. Speed ​​test agents malachite green box (Ⅰ) is only used for preliminary screening of suspected samples using the malachite green; C, malachite green speed test agent box (Ⅱ) to confirm whether the samples contain malachite green component.

Test principle

Enriching column can concentrate and enrich hundred times of the Malachite green content in the breed aquatics water, after the malachite green enriched by the enriching column, the adsorption in the column will be some obvious green ribbon. Then the malachite eluted by the eluant again. If the eluant appears green that can be used malachite green. If compare the eluant with the malachite standard sample to be characterized, to confirm whether the use of malachite green.

Specification: 10 times.

Storage: normal temperature, avoid from sunlight.

Packing: 1. malachite green enrichment column: 10 branches, 2. eluent: 2 bottles, 3. the control solution of malachite green: 1 bottle, 4. ear cleaning balls: 1 (provide for oneself), 5. Transfusion system: 4 pay.

Period validity: 6 months.

Usage

1. Sampling: First close the transfusion device at the lower end of the transfusion pipe flow controller, using 25ml or 100ml syringe sampling and want to check the fluid directly from the infusion bag ( tank ) sample adding holes to join. When the enriching column enrichment of 100ml samples can be detected malachite green minimum concentration is 3UG / L, 300ml enrichment can be detected malachite green the lowest concentration of 1 ug / L. After the sample is loaded into the transfusion bag (tank) hanging on a nail or hook.

2. Enrichment: (1) Exhaust: According to the experimental setup, first slowly open the infusion flow rate controller, so that the liquid from the drainage guide Tube out until the bottom of the liquid outflow from the catheter side by side under a clean air-water separator tube in the bubble (the liquid should be filled with gas-water separator below the catheter and the catheter should be absolutely no air bubbles), quick adjust water flow rate controller is switched off, rapidly the enrichment column big mouth on the end of the receiving drainage catheter. Accordance with the "speed test case for use of malachite green," photos two of the "Exhaust" port up under the enrichment column, and then open the flow controller, so that slowly filled with liquid enrichment column, and the discharge column gas. (2) Such as "malachite green-speed measuring box for use" as shown in photo one, put down the enrichment column, under the port, so that the liquid Flow through the enrichment column for enrichment. Control the flow rate of about 60 drops per minute.

3. Elution: flow to be completely liquid sample enrichment column, the enrichment column off; wash the ear with the ball towards the column from the column packing pressure port blowing, the water column to catch a clean and dry as possible. Enrichment column from the column after drying by adding 1 ml eluant port and let the liquid naturally shed, collecting eluate in the control vials.

4. Results of observation: If cylinder filler layer that was green and down as the eluent, the preliminary view that may contain malachite green, otherwise it is negative. Or collection of the eluate can be initially considered for the green malachite green may be.

Main technical parameters:

Flow through the enrichment column sample enrichment time to detect the lower limit of detection

      100ml             25－30min    3.0 ug／L(3.0ppb)

      300ml             75 - 90min    1.0 ug／L(1.0ppb)

Announcements

1. If the turbidity test samples should be filtered before use.

2. On-site detection of positive samples should be used to test reagents malachite green-speed box (Ⅱ) to confirm or high performance liquid chromatography.

3. The use of malachite green standard working solution: This kit provides the standard working solution available for beginners training. Standard working solution concentration is 75ug/ml, before using to draw 50mL water to adding a drop of standard working solution that has been diluted to 0.075ug/mL of malachite green solution. Take 15ml diluents through the enrichment column, column packing enriched significantly in the green.