Product introduction

Test principle

Nitrite oxidation of hemoglobin, the body can lose the ability to transport oxygen, causing chronic, acute poisoning, but also with the food, the body reacts with secondary amine to produce strong carcinogenic nitrosamine compounds. In addition to carcinogenic nitrosamines, but also through the placenta to the fetus produces teratogenic and toxic effects.

Sample application

It is widely used to detect the nitrite content of various foods

Nitrite speed test case: for test of meat and pickled vegetable (pack can be 100 times greater than the number of experiments).

Agent constitute

1. Test solution A: 2 bottles.

2. Plastic sample cup: 100 cups

3. Plastic scale straw: 100 (0.5ml)

4. Porous colorimetric tubes: 2 tubes

5. Color cards,

6. Introduction

Storage

in 4¡æ dark sealed refrigeration can be stored for 6 months, and in less than 25¡æ in a cool dry place away from light sealed storage can be stored for 3 months.

Test steps

1. with the balance and weighed 1g sample cut into small pieces as possible, into the sample cup; scale plastic straw adding 10ml distilled water or purified water, for 10 minutes, during which the straw stirring or shaking can be used to speed up the extraction, The supernatant is sample solution.

2. colorimetric tubes in porous holes in the first test liquid dropping 2 drops of A, then dropping a drop of liquid extracted samples, mixing; pause a few seconds before adding 0.5ml of distilled water (10-12 drops), shaking and mixing for 2 minutes, and then observe the color change and color card with the control.

Results judgment

After 2 minutes to observe the depth of color in the holes, the darker color indicating the higher levels of nitrite content in the samples. Control the value of the label on the color card shows roughly nitrite content of the sample.

Announcement

1. The standard working solution presented as required. It is only for the beginners to use for training. The use of standard working solution: porous than the color in the hole before dropping tube 2 drops of test solution A, and then dropping a drop of standard working solution, mix; pause a few seconds before adding 0.5ml of distilled water, shake mix. Stay for 2 minutes and observe the color change.

2. this kit used in each tube 3ml scale drip droplets of about 50ul, take 0.5m1 about 10-12 drops of liquid.

3. The reagent should be closed after use, in the refrigerator or a cool dry place away from light.

4. During sampling, the meat should be representative of the lean meat to take part of the nitrite concentration of samples exceeded the recommended repeat experiments.

5. All experiments should use distilled water or purified water.

6. After each use, all the experimental containers were washed three times more than the application of water, and then washed with distilled water or pure dry spare.

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