Product introduction

Application

This method is suitable for non edible pigment tonyred (No. 1/2/3/4) of the on-site rapid detection.

The significance of detection of Tonyred

Tonyred has the characteristic of mutagenicity and carcinogenicity, tonyred No.1 appears possible carcinogenic properties in human liver cell research. Long term food containing ¡®Tonyred¡¯ food, may cause liver DNA structural changes, leading to liver disease.

Specification: 50 times.

Storage: normal temperature, avoid from sunlight.

Packing: 1. chromatography paper: 50 papers, 2. 1 bottle of expansion agent, 3. capillary tube: 1 tube, 4. two bottles of Tonyred comparing fluid, self-service: ethyl acetate, 200ml beaker.

Period: 12 months.

Method of use

1. Take out of approximately 1 grams of sample into a test tube, adding 2 ~ 5ml ethyl acetate, shaking and extract in 1 minute, set aside for 5 minutes.

2. Take a piece of paper chromatography, in the end on the 1cm apart, parallel 0.8cm-1.0cm pencil to draw the points like several dots or painted on several ¡®+¡¯ sign.

3. With 2 capillary tubes were dipped in Tonyred (No. I, III), Tonyred (¢ò+VI) controlled liquid a little (capillary tip of not more than 0.5cm in length, distance), point in the symbol ¡®+¡¯ of paper chromatography, use another capillary dipped in ethyl acetate extracted sample solution and point it into the sample line ¡®+¡¯ of the paper chromatography, (the color of the sample solution is shallow, then can be sampling in every volatile stem after repeated sample). Spot diameter should be controlled within 2 millimeter.

4. Chromatography: take a 200ml beaker, adding about 4-5ml expansion agent, the paper chromatography (sample end down) is inserted into the expansion agent against the cup wall, to be launched agents along the paper chromatography upward mounted parallel to about 7 centimeters when removing the paper chromatography, observation results.

The results of judgment

In the experiment condition the controlled liquid chromatography results of Tonyred (No. I and III) and Tonyred (¢ò+VI) as shown in the figure. If the sample in the unfolding trajectories in spots, the spots out (up) in the distance and a control fluid expansion after the spot distance, the same shape, color is similar, can be considered a sample may join this pigment.

Announcement

1. The method can judge whether the sample into the visible Tonyred (No. 1/2/3/4), at the same time to judge whether the sample joined other inedible pigment also has the certain reference value. In case of abnormal samples available spots, high performance liquid chromatography to determine.

2. The sample application Tonyred controlled liquid sample quantity is not too much, otherwise will have spotted the trailing phenomenon. In capillary dip control fluid, can be in a waste paper chromatography with discard excess solution, so that the capillary tip have not more than 0.5 cm distance, so that the disposable to paper chromatography cross line.

3. Expansion agent should be used in proper amount, height of liquid level control should be in the spot, the unfolding process chromatography paper cannot be dumped, and each show a piece of paper chromatography is best to replace a developing agent.

4 samples more in quantity, not every paper chromatography have a point of control fluid, when the unfolding process of spots, need to repeat the experiment to control fluid.

5 Scene Detection of positive samples should be sent to laboratory confirmation, precise and quantitative needed by high performance liquid chromatography.

Note: the Tonyred controlled liquid is volatile and easy to dry, such as dry and accession to the 1-2ml ethyl acetate can be used.